CLINICAL TRIAL / NCT05356000

The study will be carried out in the University of Chicago Medicine (UCM) Clinical Research Center (CRC). Study Population and Recruitment Enroll 10 post-Roux-en-Y Gastric Bypass stone forming, 10 obese stone forming, and 10 non-obese stone forming participants over four years of the award. Patients will be recruited at the University of Chicago Kidney Stone Clinic and Bariatric Surgery Clinics (both current and repository of previous patients). The Bariatric Surgery Clinic is a large clinic and Center for Excellence for bariatric surgery. The group's four active surgeons (including study advisor, Dr. John Alverdy) perform 300 bariatric surgeries per year. Over the past 10 years, at least one quarter to one half of these procedures have been Roux-en-Y Gastric Bypass. Incidence rates for stones at 10 years after Roux-en-Y Gastric Bypass is 14% and thus, even with conservative estimates, we will have over 100 eligible patients from this clinic. Experimental Design and Implications Administration of very low oxalate diet (50mg/day) for three days followed by 13c2-oxalate and sucralose oral load test will determine the amount of oxalate absorbed via gastrointestinal (GI) paracellular pathways discriminated from endogenous oxalate production. Urine oxalate levels after low oxalate diet will provide an estimate of GI oxalate absorption combined with hepatic production (Aim 2a). By measuring the proportion of 13c2-oxalate load that appears in the urine, we will determine the fraction of absorbed oxalate that appears in the urine. Sucralose will be used with the load test as a marker of paracellular transport. Sucralose is an artificial sweetener that is absorbed in the GI tract via paracellular pathways and excreted unchanged in the urine. It is used to estimate permeability of the whole GI tract and as a marker of paracellular GI transport. Along with 13c2-oxalate, sucralose has been used to study GI oxalate transport mechanisms. The knowledge obtained from these studies will determine the contribution of GI absorption of dietary oxalate and how it is absorbed from the GI tract. These data will provide crucial information to understanding mechanisms for stone risk in obese and Roux-en-Y Gastric Bypass stone formers. If high urine oxalate in Roux-en-Y Gastric Bypass and obese stone forming is primarily due to GI hyperabsorption, then a low oxalate diet will not decrease urine oxalate levels and levels will decrease less in obese and Roux-en-Y Gastric Bypass stone formers than non-obese stone formers. This will potentially reset the clinical utility of a low oxalate diet in these patients. If high urine oxalate is from GI paracellular hyperabsorption, then the percent absorption of 13c2-oxalate and sucralose will be greater in obese and post-Roux-en-Y Gastric Bypass stone formers compared with non-obese stone formers. Follow up studies will focus on how to block or blunt this mechanism. If high urine oxalate in obese and Roux-en-Y Gastric Bypass stone forming is due to other oxalate sources (i.e. endogenous production) then the percent absorption of 13c2-oxalate and sucralose will not be greater in obese and Roux-en-Y Gastric Bypass stone formers compared with non-obese stone formers. Clinically, this will direct patients and physicians towards higher impact management strategies with less emphasis on a low oxalate diet. Future studies will investigate endogenous production of oxalate in obese and Roux-en-Y Gastric Bypass stone formers. Better strategies for oxalate management after surgery will lower kidney stone risk in these high-risk patients and lower the risk of chronic kidney disease progression and end-stage renal disease that can result from high urine oxalate. In non-obese stone formers (N=10) obese stone formers (N=10) and post-Roux-en-Y Gastric Bypass stone formers (N=10), we will measure urine oxalate and citrate before and after three days of low (50mg/day) oxalate diet. Primary endpoint (hypothesis 2a) is change in urine oxalate excretion from baseline after low oxalate diet in non-obese versus obese and Roux-en-Y Gastric Bypass stone formers. 2b and 2c: Following the low oxalate diet, give a 13c2-labeled sodium oxalate (100mg) and sucralose (5g) oral load test and measure urine oxalate, citrate, and sucralose over 24 hours. Primary endpoint (hypothesis 2b) is percent 13c2-oxalate of total administered in non-obese versus obese and Roux-en-Y Gastric Bypass stone formers. Secondary endpoint (hypothesis 2c) is percent sucralose of total administered in non-obese versus obese and Roux-en-Y Gastric Bypass stone formers. Rationale: Absorption of diet oxalate may be elevated in obese and post-Roux-en-Y Gastric Bypass stone formers, however the actual contribution of increased gastrointestinal (GI) oxalate absorption to hyperoxaluria in these patients has not been documented. It is also unclear what proportion of oxalate is absorbed via the paracellular route. If an increase in GI oxalate absorption is not sufficient to explain hyperoxaluria, it would change treatment of these patients.